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KMID : 0545120140240010013
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Journal of Microbiology and Biotechnology
2014 Volume.24 No. 1 p.13 ~ p.18
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Characterization of an Antibiotic Produced by Bacillus subtilis JW-1 that Suppresses Ralstonia solanacearum
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Kwon Jae-Won
Kim Shin-Duk
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Abstract
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Bacillus subtilis JW-1 was isolated from rhizosphere soil as a potential biocontrol agent of bacterial wilt caused by Ralstonia solanacearum. Seed treatment followed by a soil drench application with this strain resulted in >80% reduction in bacterial wilt disease compared with that in the untreated control under greenhouse conditions. The antibacterial compound produced by strain JW-1 was purified by bioactivity-guided fractionation. Based on mass spectroscopy and nuclear magnetic resonance spectral data (1H, 13C, 1H-1H correlation spectroscopies, rotating frame nuclear Overhauser effect spectroscopy, and heteronuclear multiple-bond correlation spectroscopy), the structure of this compound was elucidated as a cyclic lipopeptide composed of a heptapeptide (Gln-Leu-Leu-Val-Asp-Leu-Leu) bonded to a β-hydroxy-iso-hexadecanoic acid arranged in a lactone ring system.
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KEYWORD
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bacterial wilt, Ralstonia solanacearum, biocontrol agent, antibacterial compound, cyclic lipopeptide, surfactin isoform
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